Journal: Molecular Metabolism
Article Title: A distinct vagus-beta cell neural circuit senses glucose and modulates insulin secretion
doi: 10.1016/j.molmet.2026.102371
Figure Lengend Snippet: Changes in the vagal transcriptome representing metabolic state and diet dependent interactions . (A) Experimental schematic showing three distinct metabolic states: non-fasted (ad libitum food), fasted (no food for 18 h), and feeding (no food for 18 h followed by ad libitum food for 20 min); two distinct diets: regular chow, and high fat diet (60%); and the mouse nodose ganglion used for RNA sequencing. The water supply is ad libitum in all the groups. Each group comprises three males and three females, a total of six mice per group; (B) Plots showing impairment in glucose clearance (glucose intolerance) after four weeks on 60% high fat diet (HFD) in mice used for RNAseq (upper panel), and significantly higher area under the curve (AUC) in a glucose tolerance test for mice feed with 60% HFD for four weeks compared to age and sex-matched mice on regular chow (lower panel); (C) Pathway enrichment analysis using KEGG database shows metabolic pathway with the maximum number of genes (1,384) and with P -value, 2.11E-6 and enrichment score, 13.07 as shown in the two-dimensional scatter plot; (D) Nodose ganglion transcriptome shows metabolic state dependent changes as revealed in principal component analysis (PCA) plot. The changes are more prominent in the non-fasted group compared to fasted and feeding groups; (E) Nodose ganglion transcriptome shows diet dependent changes as revealed in principal component analysis (PCA) plot. The changes are more prominent in male mice fed with 60% high fat diet for four weeks compared to female mice fed with 60% high fat diet for four weeks or mice fed with regular chow. Data are mean ± SEM (B). P values by two-way RM ANOVA with Geisser-Greenhouse correction and Šídák’s multiple comparisons test (B, upper panel), and unpaired t test two-tailed (B, lower panel) are shown. Source data are provided in NCBI Gene Expression Omnibus (GEO) under accession number GSE281091 (C–E) and (B).
Article Snippet: The RNA sequencing data were deposited to the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) as GSE281091 .
Techniques: RNA Sequencing, RNA sequencing, Two Tailed Test, Gene Expression
